WebNov 22, 2011 · Cell counting was performed as follows: (1) after a full digestion of Cultispher-S by trypsin and using trypan blue exclusion counting method, as in [ 2 ]; (2) by crystal violet staining and nuclei counting for Cytodex-3, as in [ 2 ]; or (3) cell counting on Cytopore-2 was performed using MTT according to [ 3 ]. WebJan 28, 2024 · By using a porous gelatin micro-carriers—Cultispher-S particles in a wave-bioreactor system, the in vitro culture could be controlled for several parameters, such as temperature, pH and dissolved oxygen concentrations and rocking speed to optimize the human MSCs expansion.
Cultispher-S microcarriers support expansion of …
WebCultispher ® S MCs were sterilized according to the manufacturer’s instructions. MCs were placed in the wells or molds, and rBM-MSCs cell suspension was added on top of the MCs. Cells were kept in a 37 °C, 5% CO 2 humidified incubator. Cell medium was replaced every 2–3 days, for a total culture period of 21 days. 2.5. WebIn this study, the composite nerve guides were comprised of poly(caprolactone) (PCL) and porous collagen-based beads (CultiSphers). The incorporation of the collagenous beads results in enhanced cortical neuron adhesion, viability, and neurite extension as … maury povich fancast
Decellularized adipose tissue microcarriers as a dynamic ... - PubMed
WebCultiSpher microcarriers are available in three varieties: CultiSpher G, CultiSpher GL, and Cultispher S. All CultiSpher microcarriers are supplied as a dry powder and must be … WebMar 21, 2024 · The three microcarriers with the highest attachment efficiencies, Cytodex 3, Cytodex 1 and Cultispher S, were then used to expand the cells in 10 mL bioreactors run at 60 rpm over an 8 day period. Figure Figure2b 2 b shows images of the eCB-MSCs on Cytodex 1, Cytodex 3 and Cultispher S at Day 5. By Day 4, the cells had fallen off the … WebKey features of CultiSpher-S include: Large surface area for cell attachment Minimal seeding density Maximum number of recoverable viable cells in each scale-up step … maury povich free episodes