Webinformation. Other selection vectors can be used. • Antibiotic selection: To select for S2 cells that have been stably cotransfected with pCoHygro and a DES™ expression vector, we generally use 300 μg/mL hygromycin-B. For S2 cells stably cotransfected with pCoBlast and a DES™ Drosophila Schneider 2 (S2) Cells User Guide Web13 sep. 2024 · The present invention provides a method for manufacturing an artificial polypeptide fiber, the method comprising: a step for bundling a plurality of raw material fibers containing an artificial polypeptide; and a step for bringing an obtained bundle of raw material fibers into contact with a composition containing a binder and an enzyme.
GATEWAY Binary Vector (pGWB) - Shimane U
WebHygromycin B. 选择性抗生素. Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S … Web22 jul. 2024 · The tags of pcDNA3.1 vectors. l DYK tag: A short peptide composed of 8 amino acids (DYKDDDDK), which can be fused to the N-terminus or C-terminus of the target protein and used for the immunoadsorption purification of recombinant protein. The molecular weight of the DYK tag is small and it will not affect the biological activity of the … buy a schwinn tank
Addgene: pAG32
Web21 sep. 2024 · To express Cas9 and the guide RNA from the same autonomously replicating vector using hygromycin as a selection marker for fungal transformation, plasmid … WebThis project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical … Web5 jan. 2000 · Subsequently, a vector solution containing 106 infectious particles, ... The next day one half of the plates were subjected to hygromycin selection (selection for A431 cells), ... celebrity bathtub deaths