Web6. Pipet 10 μl of THP1-Dual™ cell culture medium per well into a 96-well white (opaque) or black plate, or a luminometer tube. 7. Prime the injector with QUANTI-Luc™ 4 Reagent working solution and proceed with the measurement. RELATED PRODUCTS Product Description Cat. Code Blasticidin Selection antibiotic ant-bl-1 Webmedium. • Spin cells down for 5 min at 200 x g at room temperature (RT). • Discard supernatant, resuspend cells in 20 ml pre-warmed complete cell culture medium and transfer cells into one T75 cell culture flask. • Incubate at standard growth conditions overnight (ON). • Check cell concentration the next day.
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WebTHP1-Dual cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs. THP1-Dual cells allow the simultaneous study of the … WebMar 1, 2024 · 2.2. Cell culture. THP-1 cells (ECACC, Salisbury, UK) were counted and seeded at a concentration of 300,000 cells/ml in antibiotic-free RPMI media +10% FCS (Sigma-Aldrich, St.Louis, MO) and maintained at 37 °C, 5% CO 2 in a humidified tissue culture incubator. THP-1 cells underwent mycoplasma testing as a quality control measure, and … taste of home caramel pecan rolls
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WebApr 30, 2024 · HAECs were maintained using a Microvascular Endothelial Cell Growth Medium-2 BulletKit from Lonza, USA. THP-1 cells were cultured in DMEM media containing 10 % fetal serum. HAECs, grown to 90 % confluency in 10 cm plates, were exposed to a Teflon cone with directional changes of flow at 1 Hz cycle (± 5 dyn/cm 2 ) for OSS in the … WebWild-type cell lines are sold with knockout cell lines only - not available for individual purchase. Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. Culture medium: RPMI + 10% FBS + 0.05 mM β-mercaptoethanol Initial handling guidelines: Upon arrival, the vial … WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … The Credible Conversations blog shares the science and innovation from inside … Certain materials in the collection may require one or more permits to be filled … Simply add the contents of the kit into your basal medium and conditioned medium, … taste of home carmelitas