2024 Top-flash assay

Top-flash assay

Author: nvjv

August undefined, 2024

WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold … WebTOP-Flash was later modified by Upstate Biotechnology to contain three TCF response elements upstream of a minimal thymidine kinase (TK) promoter. The TOPFlash reporter system provided a reliable assay of pathway activation and was crucial for the identification and characterization of several pathway components.

TCF Reporter Plasmid Kit 17-285

WebJournal: PLoS ONE Article Title: The natural compound Jatrophone interferes with Wnt/β-catenin signaling and inhibits proliferation and EMT in human triple-negative breast … WebLuciferase assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full … john hamblin play school

TOPFlash(报告基因质粒)(D2501-1μg)

WebGeneral description. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed … Web8. jan 2024 · Human embryonic kidney 293 (HEK293) cells are one of the most common cell lines used for research purposes. This article examines why they are so popular. john hamby sermons

Technology - Super-Top-Flash Luciferase Reporter Cell Line for ...

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WebLight source Xenon flash lamp Sensitivity/dynamic range Fluorescence intensity, top reading: < 0.4 fmol fluorescein/well, > 6 decades, 384-well plate Fluorescence intensity, bottom reading: < 4 fmol fluorescein/well, > 5.5 decades, 384-well plate Time-resolved fluorescence, top reading: < 120 amol Europium/well, > 6 decades, 384-well plate ... WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … john hamer book of mormonWebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay ... interbasics リーガル

"WebPočet riadkov: 10 · TOPflash (TCF Reporter Plasmid) 21-170 Sigma-Aldrich TOPflash (TCF … " - Top-flash assay

Top-flash assay

Designing a Bioluminescent Reporter Assay: Normalization

Web1. aug 2024 · How does a TOPflash/FOPflash assay work to detect beta-catenin protein expression? molecular-biology lab-techniques biotechnology. 23,081. From what I can … Web5. dec 2024 · TOP-Flash contained a wild type LEF/TCF-binding site, while FOP-Flash contained a mutant LEF/TCF-binding site. HCT116 cells were transfected with reporter constructs (TOP-Flash or...

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WebTop和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是突变的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP值来表示 … Web13. apr 2024 · Numerous studies have demonstrated the normal tissue-sparing effects of ultra-high dose rate ‘FLASH’ irradiation in vivo, with an associated reduction in damage burden being reported in vitro. Towards this, two key radiochemical mechanisms have been proposed: radical–radical recombination (RRR) and transient oxygen …

Web26. dec 2024 · 具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。数据处理：通常以TOP/FOP的比值表示。公式如下：转染 … Web(D) Relative luciferase activity in U87 cells co-transfected with miR-NC or miR-137 and the reporter vector TOP-Flash or its mutant FOP-Flash. Luciferase assay was performed to determine the transcriptional activity of β-catenin. (E) The expression levels of LRP6 in normal brain and GBM tissues.

Web8. dec 2024 · The apparent reduction upon flash-freezing first in liquid nitrogen is not statistically significant. ... For transcriptional assays, it may be best to stick to an earlier time point such as 42 hours to ensure that the secreted proteins report on events at the peak of transient intracellular expression. WebSet of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays. TCF Reporter Plasmid Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA References View Products on Sigmaaldrich.com 17-285 1 kit

WebLuciferase assay of LNCaP HASOX4 stable cell line co-transfected with 40 ng of TK-Renilla with either 500 ng of TOP-flash or FOP-flash plasmids. Luciferase activity was increased …

WebBCA Protein Assay Basis for the Assay: Quantitation of total protein content is a measurement common to many applications in basic science and clinical research. The basis for this assay is the reduction of copper in alkali by protein (biuret reaction) in the presence of the chromogenic agent bicinchoninic acid. interbasin compact committeeWeb16. máj 2024 · Super-Top-Flash Luciferase Reporter Cell Line for Canonical Wnt Signaling Case ID: C15850 Disclosure Date: 5/16/2024 Unmet Need Canonical Wnt signaling, a … inter batchWeb5. dec 2024 · TOP-Flash contained a wild type LEF/TCF-binding site, while FOP-Flash contained a mutant LEF/TCF-binding site. HCT116 cells were transfected with reporter … john hamer websiteWebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF binding sites that were cloned into the pGL3 vector (Promega), which contains a minimal promoter followed by a firefly luciferase open reading frame. john hamblin preacherWeb29. apr 2024 · TOP-Flash is a TCF-reporter plasmid, along with FOP-Flash enables quantitation of the Wnt/β-catenin signaling in cells transfected with these constructs. TOP/FOP-Flash (Genechem) was cotransfected into cells with NC mimics, miR-543 mimics, miR-543 + pcDNA3.1, miR-543 + MAPK1. john hamburg directorWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : … john hamer booksWeb30. jún 2024 · The TOP-Flash assay was carried out using STF/293 cells, which are 293 human embryonic kidney (HEK) cells stably transfected with the SuperTOP-Flash reporter construct containing a reporter ... john hamer attorney